H2N2V1, Main, Exploration, bibRecord, 001875

Identification and subtyping of avian influenza viruses by reverse transcription-PCR

Identifieur interne : 001875 ( Main/Exploration ); précédent : 001874; suivant : 001876

Identification and subtyping of avian influenza viruses by reverse transcription-PCR

Auteurs : Ming-Shiuh Lee [Taïwan] ; Poa-Chun Chang [Taïwan] ; Jui-Hung Shien [Taïwan] ; Ming-Chu Cheng [Taïwan] ; Happy K. Shieh [Taïwan]

Source :

Journal of Virological Methods [ 0166-0934 ] ; 2001.

RBID : ISTEX:5D2576E3791CB87E4885E44FE07686427B2B838B

English descriptors

KwdEn :
- Avian influenza virus, Hemagglutinin, Reverse transcription-polymerase chain reaction (RT-PCR), Subtype.
Teeft :
- A6ian 6irus, Accession numbers, Avian, Avian virus, Avian viruses, Blast search, Clin, Embryonated eggs, Equine origins, Genbank, Hemagglutinin, Homogenate, Hong kong, Microbiol, Nucleotide sequence, Nucleotide sequences, Organ homogenates, Primer, Primer design, Reference center, Reference strains, Sequence analysis, Sequence comparison, Subtype, Subtypes, Subtyping, Veterinary medicine, Virol, Virological, Virological methods, Virus.

Abstract

Abstract: Avian influenza viruses have 15 different hemagglutinin (HA) subtypes (H1–H15). We report a procedure for the identification and HA-subtyping of avian influenza virus by reverse transcription-PCR (RT-PCR). The avian influenza virus is identified by RT-PCR using a set of primers specific to the nucleoprotein (NP) gene of avian influenza virus. The HA-subtypes of avian influenza virus were determined by running simultaneously 15 RT-PCR reactions, each using a set of primers specific to one HA-subtype. For a single virus strain or isolate, only one of the 15 RT-PCR reactions will give a product of expected size, and thus the HA-subtype of the virus is determined. The result of HA-subtyping was then confirmed by sequence analysis of the PCR product. A total of 80 strains or isolates of avian influenza viruses were subtyped by this RT-PCR procedure, and the result of RT-PCR gave an excellent (100%) correlation with the result of the conventional serological method. The RT-PCR procedure we developed is rapid and sensitive, and could be used for the identification and HA-subtyping of avian influenza virus in organ homogenates.

Url:

https://api.istex.fr/ark:/67375/6H6-JZ2VDGG3-Z/fulltext.pdf

DOI: 10.1016/S0166-0934(01)00301-9

Affiliations:

Taïwan

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: Avian influenza viruses have 15 different hemagglutinin (HA) subtypes (H1–H15). We report a procedure for the identification and HA-subtyping of avian influenza virus by reverse transcription-PCR (RT-PCR). The avian influenza virus is identified by RT-PCR using a set of primers specific to the nucleoprotein (NP) gene of avian influenza virus. The HA-subtypes of avian influenza virus were determined by running simultaneously 15 RT-PCR reactions, each using a set of primers specific to one HA-subtype. For a single virus strain or isolate, only one of the 15 RT-PCR reactions will give a product of expected size, and thus the HA-subtype of the virus is determined. The result of HA-subtyping was then confirmed by sequence analysis of the PCR product. A total of 80 strains or isolates of avian influenza viruses were subtyped by this RT-PCR procedure, and the result of RT-PCR gave an excellent (100%) correlation with the result of the conventional serological method. The RT-PCR procedure we developed is rapid and sensitive, and could be used for the identification and HA-subtyping of avian influenza virus in organ homogenates.</div>
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Identification and subtyping of avian influenza viruses by reverse transcription-PCR

Identification and subtyping of avian influenza viruses by reverse transcription-PCR

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri